goat anti psa polyclonal antibody  (R&D Systems)

Journal: Lab on a chip

Article Title: Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles.

doi: 10.1039/d4lc00346b

Figure Lengend Snippet: Fig. 1 (A) Principle of MB-bound immunocomplex sample preparation. Top: The MBs conjugated with the capture antibody are mixed with a solution of PSA. Middle: Adding a biotinylated anti-PSA antibody. Bottom: Adding streptavidin-modified UCNPs. MB-bound immunocomplexes are then resuspended and transferred to a microtiter plate (analog detection) or microfluidic chip (digital detection). (B) Optical setup for imaging UCNPs in the microfluidic channel. The microfluidic channel is formed between the cover glass (thickness of 170 μm) and the PDMS mold. The excitation laser (976 nm, red arrow) is introduced by a collimator (focal length of 4.3 mm), which is connected to the tip of the optical fiber (diameter of 105 μm). A long-pass filter removes short wavelengths from the laser beam. A 900 nm short-pass dichroic mirror reflects the beam through the microscope objective (focal length of 3.3 mm, oil immersion) into the dispersion of MBs (brown circles) and UCNPs (green hexagons). Only UCNPs in the focal plane (dashed square) are displayed (highlighted hexagons). The objective collects UCNP emission (green arrow). An 875 nm short-pass filter protects the camera from excitation wavelengths. A tube lens (focal length of 200 mm) projects the emissions into the camera. (C) Photograph of the microfluidic channel molded of PDMS attached to a 170 μm thick glass slide. The rectangular cross-section of the channel had 200 μm in width and 30 μm in depth. Created with https://BioRender.com.

Article Snippet: Goat anti-PSA polyclonal antibody (AF1344) and biotinylated goat anti-PSA polyclonal antibody (BAF1344) were obtained from R&D Systems (USA).

Techniques: Sample Prep, Modification, Imaging, Microscopy, Dispersion

Journal: Communications engineering

Article Title: Glowstick-inspired smartphone-readable reporters for sensitive, multiplexed lateral flow immunoassays

doi: 10.1038/s44172-023-00075-2

Figure Lengend Snippet: a Schematic showing anti-hCG antibody-functionalized glow reporters bound to hCG and captured by anti-hCG polyclonal antibodies on the test line (TL) and unbound glow reporters captured by anti-mouse polyclonal antibodies on the control line (CL). b LFA strips for the detection of serially-diluted hCG protein with 40 nm anti-hCG-gold reporters (top) and with anti-hCG blue fluorescent reporters excited using optical excitation (middle) and using glow chemistry (bottom, inverted blue channel image). The LFA strips with blue fluorescent particles were excited optically and imaged using a Fluorchem camera. The same strips were then chemi-excited with glow chemistry and imaged inside a 3D-printed dark box with a Samsung Note 8 camera with an 8-s exposure. Gold LFA strips were imaged with a Samsung Note 8 camera under ambient light. c Intensity profiles along the length of the LFA strips shown in (b) were extracted with LFA image analysis software developed in-house; values for the integrated area under the curve for each peak (TL and CL) are shown. d Average glow LFA intensity ratios (integrated area ratio: TL/CL) ± 1 Std Dev at different hCG concentrations ( n = 3); the blue circles represent the individual value for each replicate. The dashed line denotes the average of blank + 3 Std Dev of blank and the dotted line denotes the linear correlation from 0 pg/mL to 156 pg/mL ( R = 0.96). The limit of detection is estimated to be 39 pg/mL hCG and the limit of quantitation is estimated to be 156 pg/mL hCG.

Article Snippet: However, for multiplex LFA strips, the test line contained a mixture of 3 antibodies: antibody 1 : goat polyclonal anti-hCG-α antibodies (Arista; #ABACG-0500) at 1.0 mg/mL in 1× PBS, antibody 2 : mouse anti-SARS-CoV-2 antibodies (Bioss; #bsm-41411M) at 1.0 mg/mL in 1× PBS and antibody 3 : goat polyclonal anti-PSA antibodies (R&D systems; AF1344) at 0.3 mg/mL in 1× PBS.

Techniques: Control, Software, Quantitation Assay

Journal: Communications engineering

Article Title: Glowstick-inspired smartphone-readable reporters for sensitive, multiplexed lateral flow immunoassays

doi: 10.1038/s44172-023-00075-2

Figure Lengend Snippet: a Schematic showing anti-SARS-CoV-2 nucleoprotein (NP) antibody-functionalized glow reporters bound to SARS-CoV-2 NP and captured by anti-SARS-CoV-2 NP antibodies on the test line and unbound glow reporters captured by anti-mouse polyclonal antibodies on the control line. b RGB and inverted blue channel images showing glow LFA detection of 100 pg/mL SARS-Cov-2 NP spiked into nasal swab extract (in lysis buffer). Strips were imaged inside a 3D-printed dark box with a Samsung Note 8 camera. c Intensity profiles along the lengths of LFA strips shown in ( b ); calculated areas under the curve for each peak are shown.

Article Snippet: However, for multiplex LFA strips, the test line contained a mixture of 3 antibodies: antibody 1 : goat polyclonal anti-hCG-α antibodies (Arista; #ABACG-0500) at 1.0 mg/mL in 1× PBS, antibody 2 : mouse anti-SARS-CoV-2 antibodies (Bioss; #bsm-41411M) at 1.0 mg/mL in 1× PBS and antibody 3 : goat polyclonal anti-PSA antibodies (R&D systems; AF1344) at 0.3 mg/mL in 1× PBS.

Techniques: Control, Lysis